why have a stock solution

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How do you prepare a stock solution?

• A very accurate standard alkaline solution cannot be prepared using sodium hydroxide as the primary standard. ...
• Pure anhydrous sodium carbonate, Na 2 CO 3, is used to prepare a primary standard alkaline solution.
• Solid organic acids such as oxalic acid, H 2 C 2 O 4 .2H 2 O, is used to prepare primary standard acidic solution.

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How do you calculate stock solution?

• Molarity = moles solute/Liter solution
• Molarity = 0.15 moles of KMnO 4 /0.75 L of solution
• Molarity = 0.20 M

How to make a stock solution?

How to calculate the log2 fold change?

1. What I did was right?
2. If I plot a graph what should I mention in y-axis?
3. Is there any other better way to calculate the gene expression results better?
4. How to calculate log2 fold change and does it helps to see the results more clearer?

How to prepare a solution from stock solution?

Example of How to Prepare a Solution

• Weigh out 58.44 g NaCl.
• Place the NaCl in a 1-liter volumetric flask.
• Add a small volume of distilled, deionized water to dissolve the salt.
• Fill the flask to the 1 L line.

What is one of the benefits of using stock solutions?

Stock solutions can save a lot of time, conserve materials, reduce needed storage space, and improve the accuracy with which we prepare solutions and reagents.

What is stock solution and advantages?

A stock solution is a known concentrated solution that is often times diluted to some lower concentration for laboratory use. Using a stock solution has several advantages, these include: Reduced storage space: a single stock solution can be prepared and stored rather than storing several vials of working solutions.

What is stock solution in molecular biology?

A solution of a reagent, at a stable or convenient concentration, from which appropriate dilutions can be made at the time of use. From: stock solution in Oxford Dictionary of Biochemistry and Molecular Biology »

Why is a dilute solution important?

What is the purpose of dilution? A dilution can be performed not only to lower the concentration of the analyte that is being tested, so that it is in range, but also to help eliminate interferences from other substances that may be present in the sample that can artificially alter the analysis.

Which is the major benefit of made to stock system?

The advantages of make to stock include the following: Resource Capacity Planning - When you are relying on sales and demand forecasts for your production, you are able to effectively plan your resources and production to ensure that items are being produced in the most efficient manner.

What are stock solutions give some examples?

Examples of stock solutions are a five molar solution of NaCl or two molar solution of Tris-HCl. It is important to understand that when you are diluting a solution, you are not removing any of the solute. The total amount, or mass, of the solute does not change.

What is the difference between stock solution and standard solution?

The key difference between stock solution and standard solution is that stock solution is a highly concentrated solution, whereas standard solution is a solution having a precisely known concentration. Stock solution and standard solution are related terms because standard solutions often come as stock solutions.

What is stock solution in plant tissue culture?

Volume of Stock Solution = (Desired Hormone Conc. X Medium Volume) / Stock Solution Conc. Plant Cell Culture Tested Auxins are generally used in plant cell culture at a concentration range of 0.01-10.0 mg/L.

What is the difference between stock solution and working solution?

The stock solution is the DMSO solution in which your primers are shipped. Working solutions are any dilutions that are made using the stock, generally into aqueous solution.

What is stock solution preparation?

A stock solution is prepared by weighing out an appropriate portion of a pure solid or by measuring out an appropriate volume of a pure liquid, placing it in a suitable flask, and diluting to a known volume. Exactly how one measure's the reagent depends on the desired concentration unit.

Why is it important for solutions to be diluted before they are run through spectroscopy?

Dilute solutions are prepared so as to allow a significant amount of light to pass through the solution and be measured by the recorder. Substances whose absorption is very large may have to be diluted in order for significant light to reach the recorder.

What advantage is there in diluting the original solution for the analysis?

What advantage is there in diluting the original solution for analysis? The major advantage of using an aliquot is that it makes the titration more effective by conserving both time and materials.

What is a Stock Solution?

A stock solution is a highly concentrated solution . These solutions are very useful because we can dilute a portion from the stock solution to get a desired concentration. These stock solutions are important in saving preparation time of chemical reagents. Moreover, it helps us to conserve material. This means using a stock solution to get a low concentrated solution consumes only the portion from the stock and solvent that is needed for the dilution process. It is also important to reduce the storage space because we don’t need to prepare the solution using different reagents via sophisticated methods; we only need to dilute the stock solution. In addition, it improves the accuracy of experiments.

What is the Difference Between Stock Solution and Standard Solution?

The key difference between stock solution and standard solution is that stock solution is a highly concentrated solution, whereas standard solution is a solution having a precisely known concentration. Moreover, a stock solution can be a large volume of a highly concentrate solute that can be any chemical reagent, but standard solution contains a certain chemical element or compound at highly precise concentration.

What are the two types of standard solutions?

There are two types of standard solutions as primary standards and secondary standards . A stock solution can be either a primary or a secondary standard, or it can be some other chemical reagent as well.

What is secondary standard?

A secondary standard is a reagent that has been standardized using a primary standard. The major application of primary and secondary standards is to determine the concentration of an unknown reagent, mainly in titration processes. These are some important properties of standard solutions:

What is Madhu's degree?

Madhu is a graduate in Biological Sciences with BSc (Honours) Degree and currently persuing a Masters Degree in Industrial and Environmental Chemistry. With a mind rooted firmly to basic principals of chemistry and passion for ever evolving field of industrial chemistry, she is keenly interested to be a true companion for those who seek knowledge in the subject of chemistry.

What is a dilute solution?

A dilution is a solution made by adding more solvent to a more concentrated solution (stock solution), which reduces the concentration of the solute. An example of a dilute solution is tap water, which is mostly water (solvent), with a small amount of dissolved minerals and gasses (solutes).

Why do you start with a concentrated solution and then dilute it to make a dilution?

The primary reason you start with a concentrated solution and then dilute it to make a dilution is that it's very difficult—and sometimes impossible—to accurately measure solute to prepare a dilute solution, so there would be a large degree of error in the concentration value.

Can you dilute a solution with too much solvent?

It's a common mistake to add too much solvent when making the dilution. Make sure you pour the concentrated solution into the flask and then dilute it to the volume mark. Do not, for example, mix 250 ml of concentrated solution with 1 liter of solvent to make a 1-liter solution.

Who is Anne Marie Helmenstine?

in biomedical sciences and is a science writer, educator, and consultant. She has taught science courses at the high school, college, and graduate levels. our editorial process. Facebook Facebook. Twitter Twitter. Anne Marie Helmenstine, Ph.D.