Why is agar slant used?
Slanting the surface of the agar gives the bacteria a greater surface area on which to grow in a test tube. Furthermore, slants are created in test tubes that can be capped, which minimizes water loss.
What factors influence the appearance of microbial colony quizlet?
What factors influence the colony morphology of a microbial growth? All above( Genetic makeup, nutrient availability, temperature, incubation time.)
When describing the growth characteristics of a colony which of the following must be included?
Three critical aspects of a description of bacterial growth are colony size, color, and shape.
What is flocculent in microbiology?
Flocculent – small masses are floating at the surface.
What factors influence the appearance of microbial colony?
In general, as environmental conditions become less favorable, the pattern of growth in a colony becomes more complex [4]. Two of the main factors that affect bacterial growth in a laboratory are media nutrient density and media hardness, the latter being a result of agar concentration.
What is the significance of describing the cultural or colonial characteristics of bacteria?
Although one might not necessarily see the importance of colonial morphology at first, it really can be important when identifying the bacterium. Features of the colonies may help to pinpoint the identity of the bacterium. Different species of bacteria can produce very different colonies.
Are bacteria easier to observe on a plate or in a broth?
How do the bacteria look on the plate as compared to the broth? Bacteria are easier to observe on a plate than on a broth.
What makes agar a good support for microbial growth?
Agar is an ideal solidifying agent for microbiological media because of its melting properties and because it has no nutritive value for the vast majority of bacteria. Solid agar melts at about100°C; liquid agar solidifies at about 42°C.
Which method can be used to maintain the culture for a longer period of time?
Freeze-drying is the preferred method keeping reference strains and other isolates for long periods and is standard practice for large commercial and national culture collections.
Where do bacteria grow in an agar slant?
On agar plates, bacteria grow in collections of cells called colonies.
What are broth cultures used for?
Broth cultures are a method of growing bacteria in a liquid growth medium. They're used to grow and maintain cultures for a laboratory.
What is the purpose of using a broth medium when collecting bacteria from surfaces?
BROTH: liquid medium made with various nutrients and indicators. Allows for the growth of large volumes of bacteria, the level of growth can be assessed based on the turbidity (cloudiness) of the culture. Bacteria are inoculated into a broth using a loop.
What is a culture medium?
A culture medium is a solid, semi-solid, or liquid substrate in order to support the growth of microorganisms or cells. Two major types of growth media are cellular growth media for cell cultures, and bacterial growth media for microbiological cultures. The most common growth media in terms of microbiological cultures consists of nutrient broths or agar plates, with some specialized media for biochemical identification of bacteria.
Can bacteria grow in a lab?
Despite the fact that bacteria are growing all around you, they can be surprisingly difficult to grow in a lab. This lesson will explore some of the basics involved in culturing bacteria for experimentation.