Full Answer
What is the purpose of aliquoting stock reagents?
Aliquoting stock reagents into smaller volumes also helps preserve the stability of the stock reagent by reducing the number of freeze-thaw cycles of the entire stock. Label the new containers with the contents, the date it was aliquoted, and expiry date, if applicable.
What is a reagent?
Reagents may be compounds or mixtures. In organic chemistry, most are small organic molecules or inorganic compounds. Examples of reagents include Grignard reagent, Tollens' reagent, Fehling's reagent, Collins reagent, and Fenton's reagent.
What reagent should I use to prevent source contamination?
Aliquot stock reagents (e.g. serum, trypsin, etc.) to ensure you have fresh, uncontaminated supplies. Whether or not it’s a shared reagent, pre-measured aliquots are an effective way to reduce the chance of source contamination.
How do you organize your reagents and reagents?
Have a discussion with your lab mates to determine which commonly used reagents should be kept within arm's reach on the lab bench and which rarely used reagents can be stored away. You can also reduce clutter by creating a schedule for going through your reagents and removing expired and obsolete reagents from your storage space.
What is a stock solution in biology?
A solution of a reagent, at a stable or convenient concentration, from which appropriate dilutions can be made at the time of use. From: stock solution in Oxford Dictionary of Biochemistry and Molecular Biology »
What is an example of a stock solution?
Examples of stock solutions are a five molar solution of NaCl or two molar solution of Tris-HCl. It is important to understand that when you are diluting a solution, you are not removing any of the solute. The total amount, or mass, of the solute does not change.
What is a stock standard in the laboratory?
Solutions containing a precise mass of solute in a precise volume of solution are called stock (or standard) solutions. To prepare a standard solution, a piece of lab equipment called a volumetric flask should be used. These flasks range in size from 10 mL to 2000 mL and are carefully calibrated to a single volume.
What is a stock solution in pharmacy?
Stock solutions are solutions of known concentration that are prepared by the pharmacist or technician for convenience in dispensing. They are usually strong solutions from which weaker ones may be made conveniently.
How do you make stock solutions?
A stock solution is prepared by weighing out an appropriate portion of a pure solid or by measuring out an appropriate volume of a pure liquid, placing it in a suitable flask, and diluting to a known volume. Exactly how one measure's the reagent depends on the desired concentration unit.
How do you find stock solutions?
Strategy:Calculate the number of moles of glucose contained in the indicated volume of dilute solution by multiplying the volume of the solution by its molarity.To determine the volume of stock solution needed, divide the number of moles of glucose by the molarity of the stock solution.
What is the purpose of a stock solution?
Stock solutions are used to save preparation time, conserve materials, reduce storage space, and improve the accuracy with which working lower concentration solutions are prepared.
What is standard and stock solution?
The key difference between stock solution and standard solution is that stock solution is a highly concentrated solution, whereas standard solution is a solution having a precisely known concentration. Stock solution and standard solution are related terms because standard solutions often come as stock solutions.
What is the difference between stock and working solution?
The stock solution is the DMSO solution in which your primers are shipped. Working solutions are any dilutions that are made using the stock, generally into aqueous solution.
How is stock solution prepared in pharmacology?
5:128:47Making Stock Solution for use in Pharmacology practicals - YouTubeYouTubeStart of suggested clipEnd of suggested clipI will then have 10 milliliters of stock and 90 milliliters of water.MoreI will then have 10 milliliters of stock and 90 milliliters of water.
How do you make a stock solution from powder?
Place 200g of SDS powder a 1L beaker and add H2O up to 900ml. When dissolved, transfer solution to a 1L graduated cylinder and add H2O to 1000ml. Mix 20ml EtOH with 80ml H2O for a final volume of 100ml.
What is a stock solution quizlet?
Stock Solution. a concentrated solution that will be diluted to a lower concentration for actual use. Dilution.
Designate a Place for Everything
Make sure everything in your lab has a specific place. Designate specific, clearly-labeled storage locations for each reagent or category of reagents to ensure that newly-delivered items can easily be found by all lab members.
Label Everything
Establish a consistent labeling system that all lab members use to label aliquots and shared solutions. Don't forget to also label shelves and cupboards, which can remind people of where items belong and help keep storage areas organized. Be sure to create clear labels using alcohol-resistant markers.
Use Spreadsheets
Keep an updated lab inventory spreadsheet and ensure all lab members update this sheet when receiving new items. A basic lab inventory spreadsheet containing reagent names and ordering information (i.e. vendor, catalog number, price, etc.) can be created using Microsoft Excel or Google Sheets.
Aliquot Stock Reagents
Aliquot stock reagents (e.g. serum, trypsin, etc.) to ensure you have fresh, uncontaminated supplies. Whether or not it’s a shared reagent, pre-measured aliquots are an effective way to reduce the chance of source contamination.
Create a Departure Checklist
Whenever someone leaves the lab, complete a “departure checklist” that includes reviewing, organizing, labeling, or discarding stored reagents . This process will minimize the accumulation of reagents stored in the lab and ensure new lab members have space to store their own supplies.
Efficient Tools and Technologies for Life Science Research
Accomplish more in less time and with less effort by making smarter choices for the tools you use in the lab, including cell isolation and cell culture technologies.
What is a stock warrant?
Stock warrants are an innovative financial instrument that give holders the right, but not the obligation, to buy a stock at a certain strike price. If that sounds like an option to you, you're right. Warrants and options are very similar, and we'll discuss the differences in this article.
Stock warrants vs. stock options
Stock options are contracts between two parties that give the holder of the option the right to buy or sell the underlying stock at a set strike price by a deadline. The seller of call options is typically required to hold the underlying stock in their portfolio for the duration of the option.
Types of stock warrants
There are different types of warrants that have subtle tweaks from the type discussed above. Let's go over the different types.
Warrants can work for some investors
Stock warrants aren't as popular in the U.S. as they once were. Stock options are popular enough that the market for warrants isn't robust, and companies are able to raise capital without the added costs of warrants. That isn't a bad thing for seasoned investors.
What does reagent grade mean?
What Reagent-Grade Means. When purchasing chemicals, you may see them identified as "reagent-grade.". What this means is that the substance is sufficiently pure to be used for physical testing, chemical analysis, or for chemical reactions that require pure chemicals. The standards required for a chemical to meet reagent-grade quality are determined ...
What is reagent in chemical testing?
Updated December 07, 2019. A reagent is a compound or mixture added to a system to cause a chemical reaction or test if a reaction occurs . A reagent may be used to find out whether or not a specific chemical substance is present by causing a reaction to occur with it.
What are some examples of reagents?
Examples of reagents include Grignard reagent, Tollens' reagent, Fehling's reagent, Collins reagent, and Fenton's reagent. However, a substance may be used as a reagent without having the word "reagent" in its name.
Is a catalyst a reagent or a reactant?
The term reagent is often used in place of reactant, however, a reagent may not necessarily be consumed in a reaction as a reactant would be. For example, a catalyst is a reagent but is not consumed in the reaction. A solvent often is involved in a chemical reaction but it's considered a reagent, not a reactant.
Why is stock solution important?
These solutions are very useful because we can dilute a portion from the stock solution to get a desired concentration. These stock solutions are important in saving preparation time of chemical reagents. Moreover, it helps us to conserve material.
What is the difference between stock solution and standard solution?
The key difference between stock solution and standard solution is that the stock solution is a highly concentrated solution, whereas standard solution is a solution having a precisely known concentration.
What is standard solution?
What is a Standard Solution? A standard solution is a concentrated solution having a precisely known concentration. In the preparation of a standard solution, we can use an accurately weighed solute and dissolve it in a suitable solvent to get a specific volume of the solution.
What Are Refreshers?
The concise definition of a refresher, according to one Quora post, is
Why Do Only Some Companies Grant Refreshers?
According to an article in Forbes, companies in the 1990s and 2000s only awarded RSU and option grants when employees were first hired. However, because of the steep drop-off in compensation after an initial grant is fully vested, this practice gives many employees little incentive to stay at their company past the four-year mark.
How Do Different Companies Handle Refreshers?
Startups can be especially hesitant to give refreshers, although many do have their own systems in place. One startup adviser estimates that only about 30-40% of startups give equity refreshers to long-term employees.