How do you prepare a 50ml solution of a solution?
As an example, say you need to prepare 50 ml of a 1.0 M solution from a 2.0 M stock solution. Your first step is to calculate the volume of stock solution that is required. So to make your solution, you pour 25 ml of stock solution into a 50 ml volumetric flask. Dilute with solvent to the 50 ml line.
What is the volume of the stock solution needed to prepare 12m?
If X is the volume of stock solution (12M) required, 12X = 0.05. X = 0.00415 liters =4.15 ml of the stock solution. Without specialize equipment, the best you can do is pipette 5.2 ml of the stock solution into a 500 ml volumetric flask.
How do you dissolve sodium chloride in milliq?
Measure out 80 mL of MilliQ water and add to the Duran bottle. Dissolve the sodium chloride by adding a magnetic flea into the bottle and placing on a magnetic stirrer. It may take a few minutes to fully dissolve.
How to calculate volume of stock solution for dilution?
Your first step is to calculate the volume of stock solution that is required. MdilutionVdilution = MstockVstock. (1.0 M)(50 ml) = (2.0 M)(x ml) x = [(1.0 M)(50 ml)]/2.0 M. x = 25 ml of stock solution. So to make your solution, you pour 25 ml of stock solution into a 50 ml volumetric flask.
Why do you start with a concentrated solution and then dilute it to make a dilution?
The primary reason you start with a concentrated solution and then dilute it to make a dilution is that it's very difficult—and sometimes impossible—to accurately measure solute to prepare a dilute solution, so there would be a large degree of error in the concentration value.
What is a dilute solution?
A dilution is a solution made by adding more solvent to a more concentrated solution (stock solution), which reduces the concentration of the solute. An example of a dilute solution is tap water, which is mostly water (solvent), with a small amount of dissolved minerals and gasses (solutes).
Can you dilute a solution with too much solvent?
It's a common mistake to add too much solvent when making the dilution. Make sure you pour the concentrated solution into the flask and then dilute it to the volume mark. Do not, for example, mix 250 ml of concentrated solution with 1 liter of solvent to make a 1-liter solution.
What is NaCl solution?
About sodium chloride solution. Sodium chloride (NaCl) solution, especially at a 5 M concentration, is a commonly used stock solution for a variety of solutions. One example of sodium chloride use is to precipitate nucleic acids during their extractions.
How to dissolve sodium chloride?
Dissolve the sodium chloride by adding a magnetic flea into the bottle and placing on a magnetic stirrer. It may take a few minutes to fully dissolve. Top up the solution to 100 mL with MilliQ water. To sterilise, autoclave the solution on a liquid cycle (20 min at 15 psi).