METHOD
- Add 5 mL of the saturated trehalose solution to a tube containing 10 mL of 4.9 M sorbitol solution.
- Using a disposable pipette, add <1 cm of Chelex 100 resin to the tube. Vortex well.
- Incubate the solution for 3 h at room temperature (25°C) without further agitation.
- Centrifuge at 2000 rpm for 10 min.
- Transfer the supernatant to a fresh tube. Store...
- Add 5 mL of the saturated trehalose solution to a tube containing 10 mL of 4.9 M sorbitol solution.
- Using a disposable pipette, add <1 cm of Chelex 100 resin to the tube. ...
- Incubate the solution for 3 h at room temperature (25°C) without further agitation.
- Centrifuge at 2000 rpm for 10 min.
What is sorbitol solution made of?
Sorbitol Solution is an aqueous solution containingnot less than 64.0 percent of D-sorbitol (C6H14O6). Theamounts of total sugars, other polyhydric alcohols, andany hexitol anhydrides, if detected, are not included inthe requirements nor in the calculated amount under
How do I make a stock solution?
Your first step is to calculate the volume of stock solution that is required. To make your solution, pour 25 ml of stock solution into a 50 ml volumetric flask. Dilute it with solvent to the 50 ml line.
How do you use chelex 100 resin with sorbitol?
Perform all procedures under RNase-free conditions. 1. Add 5 mL of the saturated trehalose solution to a tube containing 10 mL of 4.9 M sorbitol solution. 2. Using a disposable pipette, add <1 cm of Chelex 100 resin to the tube.
How do you dilute a concentrated stock solution?
Make sure you pour the concentrated solution into the flask and then dilute it to the volume mark. Do not, for example, mix 250 ml of concentrated solution with 1 liter of solvent to make a 1-liter solution. Helmenstine, Anne Marie, Ph.D. "Dilution Calculations From Stock Solutions."
How do you make 1M sorbitol solution?
Sorbitol 1MDissolve 182 g sorbitol in 800 ml ddH2O.Fill up to 1 liter.
Can we autoclave sorbitol?
It is not too much trouble to autoclave sorbitol and medium separately so I would do it.
How do you make a 5 molar potassium acetate solution?
Dissolve 14.7 g of potassium acetate (FW 98.2) in 20 ml water and bring the final volume to 30 ml to make 5 M potassium acetate.
How do you make potassium acetate solution?
Dissolve 78.5 g of potassium acetate (m.w. = 98.14) in 80 mL of H2O. Add H2O to make a total volume of 100 mL of solution. Filter-sterilize through a 0.22-μm filter. Store at room temperature (indefinitely).
What is sorbitol solution?
SORBITOL is a laxative. This medicine is used to relieve constipation. This medicine may be used for other purposes; ask your health care provider or pharmacist if you have questions.
What is the solubility of sorbitol?
Sorbitol is a white crystalline solid, with a sweet fruity taste, more pronounced than that of xylitol. It is extremely soluble in water (2350 g/L), acetic acid, ethanol and methanol, but insoluble in most nonpolar solvents. Sorbitol melts at 111 °C and boils between 290–295 °C (values altered by water content).
How do you make 1M potassium acetate solution?
1 M Potassium Acetate (KOAc, pH 7.5) Dissolve 9.82 g of potassium acetate in 90 ml of pure H2O (Milli-Q or equiv- alent). Adjust the pH to 7.5 with 2 M acetic acid. Add pure H2O to 100 ml. Divide the solution into aliquots and store them at –20°C.
How do you make 3 M potassium acetate?
3 M Potassium / 5 M Acetate To prepare 1 liter of this solution, dissolve 294.42 g potassium acetate in 100 ml water, and add glacial acetic acid until a pH of 4.6 is reached. This will require about 40-50% of the final volume to be acetic acid.
How will you prepare 0.01 M sodium acetate solution?
Acetate Buffer (0.1 M, pH 5.0) Preparation and RecipePrepare 800 mL of distilled water in a suitable container.Add 5.772 g of Sodium Acetate to the solution.Add 1.778 g of Acetic Acid to the solution.Adjust solution to desired pH using 10N HCl (typically pH ≈ 5.0).Add distilled water until the volume is 1 L.
What is the pH of potassium acetate?
Potassium Acetate (3 M), pH 5.5, RNase-free.
How much potassium is in potassium acetate?
The solution is administered after dilution by the intravenous route as an electrolyte replenisher. It must not be administered undiluted. Each mL contains 196 mg of potassium acetate which provides 2 mEq each of potassium (K+) and acetate (CH3COO¯). The solution may contain acetic acid for pH adjustment.
Does potassium acetate dissolve in water?
Potassium acetate (CH3COOK) is the potassium salt of acetic acid. It is a hygroscopic solid at room temperature....Potassium acetate.NamesSolubility in water216.7 g/100 mL (0.1 °C) 233.8 g/100 mL (10 °C) 268.6 g/100 mL (25 °C) 320.8 g/100 mL (40 °C) 390.7 g/100 mL (96 °C)41 more rows
Review of Dilution, Concentration, and Stock Solutions
A dilution is a solution made by adding more solvent to a more concentrated solution (stock solution), which reduces the concentration of the solute. An example of a dilute solution is tap water, which is mostly water (solvent), with a small amount of dissolved minerals and gasses (solutes).
Dilution Example
As an example, say you need to prepare 50 milliliters of a 1.0 M solution from a 2.0 M stock solution. Your first step is to calculate the volume of stock solution that is required.
Avoid This Common Dilution Mistake
It's a common mistake to add too much solvent when making the dilution. Make sure you pour the concentrated solution into the flask and then dilute it to the volume mark. Do not, for example, mix 250 ml of concentrated solution with 1 liter of solvent to make a 1-liter solution.
