How to calculate the number of ul of DNA in water?
That's correct, though I would just calculate (10 ng)/ (937 ng/ul) to get 0.01 ul (after rounding off). Then combine this with 15-0.01=14.99 ul of water. Since you can't pipet 0.01 ul of DNA, you should dilute your DNA at least 1 to 100 (I would put 2 ul into 198 ul) and then add 1.1 ul of this to 13.9 ul of water.
How do you dilute DNA to 198?
Since you can't pipet 0.01 ul of DNA, you should dilute your DNA at least 1 to 100 (I would put 2 ul into 198 ul) and then add 1.1 ul of this to 13.9 ul of water. Good luck. Yes, it makes sense. The calculation is ok.
How much DNA should I add to my reaction vial?
I need to add 10 ng of DNA in a reaction vial and add water for a final volume of 15 ul. I start from my DNA extract which is at a concentration of 937 ng/ul.
How do I resuspend oligonucleotides to 100 μm?
You can also use the free, online IDT Resuspension Calculator to make most of these calculations. To resuspend your oligonucleotides to 100 μM, simply multiply the number of nanomoles (nmol) by 10 to get the volume (in μL) of water or buffer to add.
How do you take 1ug of DNA?
Then, when you have your concentration, you need to calculate which volume of your sample contains 1ug of DNA. So to say, if you have 20ul of DNA with concentration of 0.25 ug/ul, you will have 1ug of DNA in 4ul of your sample.
How do you calculate DNA stock?
Answer: There are two ways to solve this problem:Calculate the total amount of DNA in the solution, then divide by the total volume: 10 µl x 4 µg/µl = 40 µg of DNA. 40 µg DNA/ 50 µl = 0.8 µg/µl.Just plug the values into the formula: (initial concentration)(initial volume) = (final concentration)(final volume)
How do you dilute 100ng UL?
Then you can dilute 1:10 to obtain 100 ng/ul solution or 1:100 to 10 ng/ul solution. (It is important because, if your stock is 100 ng/ul and you need 125 ng, you have to take 1,25 ul.
How do you make a 10mg solution of mL?
To prepare a concentration of 10 µg/ml, pipette out 10 µl of the drug in a test tube using a micropipette. Then dilute it with 990 µl (making a total volume of 1 ml) of the solvent (ethanol, methanol, water etc) you are going to use in your experiment.
What are the methods of quantification of DNA?
Comparison of DNA quantification MethodsMethodSensitivityUV spectrophotometry2 ng/µL (typical, with microvolume spectrophotometer)Diphenylamine method3 µgFluorescence measurement10-50 pg/µL (typical, depending on kit used)Electrophoresis10 ng (typical, depending on specific ladder and imaging conditions)
How do you calculate the dilution factor of DNA concentration?
To determine the concentration of DNA in the original sample, perform the following calculation:dsDNA concentration = 50 μg/mL × OD260 × dilution factor.dsDNA concentration = 50 μg/mL × 0.65 × 50.dsDNA concentration = 1.63 mg/mL.
How do you dilute DNA to Ng uL?
i.e. 400 * 5 = 2000ng & 100 * 20 = 2000ng. This means you need to do a 1:4 dilution of your sample (just like Dirk Schmidt suggested this would mean 1 part DNA to 3 parts water/buffer).
How do you dilute a stock solution?
To make a dilution, you simply add a small quantity of a concentrated stock solution to an amount of pure solvent. The resulting solution contains the amount of solute originally taken from the stock solution but disperses that solute throughout a greater volume.
How do you dilute a stock calculator?
Dilute Solution of Known Molarity The calculator uses the formula M1V1 = M2V2 where "1" represents the concentrated conditions (i.e., stock solution molarity and volume) and "2" represents the diluted conditions (i.e., desired volume and molarity).
How do you make a 10X stock solution?
To make 1 L of 10X TBS stock solution, dissolve 24 g Tris and 88 g NaCl in 900 mL of water and then adjust the pH to 7.6 and final volume to 1 L.
How do you create different concentrations from a stock solution?
You can first prepare 0.5% solution (0.5 g per 100 ml) and for 0.25, 0.1, 0.025 and 0.05%, respectively, dilute to half, one fifth, one twentieth and one tenth. For example, add 9 ml water to 1 ml of the stock solution, to 10 times dilute and obtain a concentration of 0.05%.
How can I make 10mg ml from 1mg ml?
Weigh out 10mg of the extract and dissolve in 10ml of your solvent. Now take 0.1(100ul) of your stock solution and 0.9(900ul) of your solvent, this will become 1mg/ml solution.
Making a 100 μM solution
Figure 1. To resuspend your oligonucleotides to 100 μM, simply multiply the number of nanomoles (nmol) by 10 to get the volume (in μL) of water or buffer to add. The equation on the left shows an example, assuming you have an oligonucleotide of 1 nmol final yield.
Calculating nmol
Figure 2. Use this formula to calculate nmol when only the OD (absorbance at 260 nm) and extinction coefficient are provided.
Calculating micrograms
Figure 3. Use this formula to calculate micrograms (μg) of oligo when nmol and molecular weight (g/mol) are provided.
Calculating copy number
Figure 4. Use this formula to calculate the number of copies of your DNA sequence when moles are provided. Note that 6.022 x10 23 is Avogadro's number, the number of molecules in 1 mole.
Calculating concentration
Figure 5. Use these formulas to calculate molar concentration (μM) when molecular weight (g/mol) and concentration in μg/μL are provided.
Most recent answer
Everyone has explained very well. Additionally, I think following links are very helpful in calculating accurately.
Similar questions and discussions
How do I get 200ng of oligonucleotide from 100uM oligonucleotide stock?
What genes are isolated from yeast?
A short protocol was developed that allows the rapid isolation of any known Saccharomyces cerevisiae gene. Two known genes, APN1 and IMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.
What is the PCR for GMOs?
The detection of genetically modified organisms (GMOs) by the polymerase chain reaction (PCR) is a complex multiparameter problem. Therefore, a number of critical issues in respect to quality control need to be considered.
What is Dindial Ramotar?
Dindial Ramotar. A short protocol was developed that allows the rapid isolation of any known Saccharomyces cerevisiae gene. Two known genes, APN1 and IMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.
Can you use DNA for PCR?
And, yes, your DNA would be enough for PCR. If your PCR works than you can probably dilute your DNA a million fold. i.e. 400 * 5 = 2000ng & 100 * 20 = 2000ng. This means you need to do a 1:4 dilution of your sample (just like Dirk Schmidt suggested this would mean 1 part DNA to 3 parts water/buffer).