Hi Mi, pour the concentrated TX-100 into a 50 ml conical tube to the 5 ml mark. Add PBS to the 50 ml mark and put the conical tube into an end-over-end mixer overnight. After that you have a 10% TX-100 stock solution that is much easier to handle and dilute.
How do you make Triton X-100 solution?
10% (wt/vol) Triton X-100 solution i) Dissolve 10 g of Triton X-100 (e.g., Nacalai Tesque, Code 355-01 or similar grade) in 80 ml of Milli-Q water by stirring. ii) Add Milli-Q water to make 100 ml and stir until well mixed. iii) Store at 4 ºC.
How to dilute tx-100 to 1% solution?
This is your 10 ml of 1% triton x solution. Dilute 10 times to get 0.1% solution. Hi Mi, pour the concentrated TX-100 into a 50 ml conical tube to the 5 ml mark.
Does Triton x-100-based cell separation work for M capsulatus?
The Triton X-100-based separation of the M. capsulatus cell envelope has proven to have similar effects as first observed for E. coli ( Fjellbirkeland et al., 1997; Schnaitman, 1971a,b ).
Is tx-100 stock solution a liquid or a solid?
After that you have a 10% TX-100 stock solution that is much easier to handle and dilute. Of course its a liquid, but its a very gooey one (at room temp), and its hard to use the exact amount in this state.
How do you dissolve Triton X-100?
SOLUBILITY / STABILITY: Triton X-100 is soluble in all proportions at 25°C in water, benzene, toluene, xylene, trichloroethylene, ethylene glycol, ethyl ether, ethanol, isopropanol, and ethylene di- chloride.
How do you make a 20% Triton X-100 solution?
1) Mix 20 ml of Triton X-100 with 80 ml of ddH2O by stirring. 2) Store at 4°C.
How do you dissolve Triton X in water?
Stock solution 10% (wt/vol) Triton X-100 solution i) Dissolve 10 g of Triton X-100 (e.g., Nacalai Tesque, Code 355-01 or similar grade) in 80 ml of Milli-Q water by stirring. ii) Add Milli-Q water to make 100 ml and stir until well mixed.
How much of a 10% Triton X stock solution should the researcher have taken to make 1 ml of 0.1% Triton X solution?
10 timesMost recent answer Dilute 10 times to get 0.1% solution.
Can I autoclave Triton?
Regarding Tris and EDTA, we usually sterilize them by autoclaving. As for detergents such as Triton-X, sterilization by filtration (0.22um) is your best choice.