Add 1.4286 milliliter of water to make the stock solution 2. If you can't add add the above mentioned amount of water then you can calculate the molarity again and it would be 14.2857 millimolar.
What is the best way to make a stock solution?
If you have enough of the drug compound the best way would be to make a larger stock solution. If your balance fluctuates at the 0.1 mg scale, you should weight a little over 10 mg drug and add water to get a 1.4 mg/mL solution. Use a micropipette that you are fairly certain is in good working order.
How can I prepare a 10mm drug stock solution with 125ug?
With 125ug you can make 9.96uL of a 10mM solution of your drug, which you then can use to prepare the desired dilutions for your experiments. I hope that helps. Good luck! How can I accurately prepare a 10 mM drug stock solution? I need to prepare 1.0 mL of a 10 mM drug stock solution.
How do I make a stock solution of a substance in DMSO?
How do I make a stock solution of a substance in DMSO? According to my paper I have to dissolve carvacrol and thymol in DMSO. Particularly, it says that the substances are dissolved in DMSO and diluted in culture medium. The final concentration of DMSO in cells is 0.1%.
How to make a stock solution of Org comps?
Add 1.4286 milliliter of water to make the stock solution 2. If you can't add add the above mentioned amount of water then you can calculate the molarity again and it would be 14.2857 millimolar. Now the final step. take 700 uL from the solution (your org comp dissolved in water) and pour it in a separate vial (2 mL) and add 300 uL of water in it.
How do you make a mM solution?
A concentration of 70 mM is the same as 0.07 moles per liter. Take 0.07 moles/liter times 342.3 grams per mole and you have 23.96 grams needed per liter. To make 200 milliliters of your solution multiply grams/liter by liters needed. Since 200 milliliters is 0.2L, multiply 23.96 grams by 0.2L to get 4.792 grams needed.
How do you make a mL from a stock solution?
To prepare the 10 mL of 2 M solution, you must first transfer about 5 mL of distilled water into your 10 mL volumetric flask. Next, slowly add your 4 mL of stock solution (sulfuric acid). Swirl the flask and then top it up with more distilled water to the 10 mL mark.
How do you make a 1M stock solution?
Molarity (M) means the number of moles of solute per liter of solution. To prepare a 1 M solution, slowly add 1 formula weight of compound to a clean 1-L volumetric flask half filled with distilled or deionized water. Allow the compound to dissolve completely, swirling the flask gently if necessary.
How do I make a stock solution?
A stock solution is prepared by weighing out an appropriate portion of a pure solid or by measuring out an appropriate volume of a pure liquid, placing it in a suitable flask, and diluting to a known volume. Exactly how one measure's the reagent depends on the desired concentration unit.
How do you make 10 mM solution from 1M?
Take 1 part of your stock solution and add 9 parts of solvent (usually water but sometimes alcohol or other organic solvent). In all cases you are diluting by the same factor. The concentration of the resulting solution is 1M /10 = 0.1M where 10 is the dilution factor.
How do you make a 2mm solution?
Method: Dilute the 50 mL of 50 mM solutiom to a final volume of 1,275 mL . Yopu now have a 2 mM solution.
How do you make a 1000X stock solution?
Preparation of 1000X Antibiotic Stock SolutionWeigh x grams of antibiotic-of-interest.Dilute in pure 100% ethanol for chloramphenicol or sterile deionized water for kanamycin and ampicillin.Syringe filter water-based antibiotic solution using a 0.22-μm filter.
How do I make a 20mm solution?
To prepare a 20 mM solution take 2.05 ml of your 30% solution and fill to 1 L with water (20 mM x 1000 ml / 9.79 M). If, however, the concentration of your stock solution is given as v/v = volume per volume, then Arvind is correct in calculating the molarity of the stock solution as 12.7 M.
How do you make 5 mg/ml stock solution?
All Answers (7) Somayeh, Dissolve 5 gram in 1000 ml : 5 mg /ml, Then 0.5 gram in 1000 ml : 0.5 mg /ml. in order to prepare the various concentration you must first prepare a stock solution of your plant extract. prepare the the stock by dissolving 0.5 gm in solvent.
How do you make a 100 mM stock solution?
For practical purpuse it is best to prepare a concentrated stock solution. 416.6 mg in 10 ml will give you 100 mM solution. This can be aliquoted into multiple vials and kept frozen. When needed, thaw one vial and then use this stock soluition to make further dilutions.
How do you make a 10 mM NaCl solution?
Weigh 10g of sodium chloride. Pour it into a graduated cylinder or volumetric flask containing about 80ml of water. Once the sodium chloride has dissolved completely (swirl the flask gently if necessary), add water to bring the volume up to the final 100 ml.
How do you make a 50 nm solution?
So, you need 10 microliters of your 10micromolar stock solution to prepare the RNAimax transfection mix. add this transfection mix to you cells that are cover with 2 ml of medium. Your concentration in this medium will be 50nm inhibitor.
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One of the bioactive compounds in this oil is Thymoquinone the molecular weight of it is : 164.20 g/mol (I have the pure powder extract >98% pure)
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You can start from the total amount of DMSO that you need. If the final concentration of DMSO has to be 0.1%, that means that, for example, you will need 100µl DMSO (0.1ml) in 100ml di medium. To be precise, you will have to take 99.9ml medium + 0.1ml DMSO.
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You can start from the total amount of DMSO that you need. If the final concentration of DMSO has to be 0.1%, that means that, for example, you will need 100µl DMSO (0.1ml) in 100ml di medium. To be precise, you will have to take 99.9ml medium + 0.1ml DMSO.
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