Step 1. Prepare a ml hygromycin stock solution ( mg ml-1) by adding. g hygromycin B. Step 2. Dissolve in ml dH 2 0 and filter-sterilize. Step 3. Make aliquots and store at 4°C.
How many milliliters of hygrogold are in hygromycin?
Hygromycin B Gold (previously named HygroGold™) is supplied as a sterile filtered yellow solution at 100 mg/ml in HEPES buffer. This product is available in two pack sizes: ant-hg-1: 10 x 1 ml (1 g) ant-hg-5: 1 x 50 ml (5 g)
What is the concentration of hygromycin B?
Gibco® Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 µg/mL. This product is supplied as a 50 mg/mL solution.
What is hygromycin B 50 mg used for?
Gibco® Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 µg/mL. This product is supplied as a 50 mg/mL solution. Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene ( hyg or hph ).
What is the preferred storage method for hygromycin B Gold?
What is the preferred storage method for Hygromycin B Gold? Hygromycin B Gold is shipped at room temperature. Upon receipt, it can be stored at 4°C for short term storage or -20°C for long term storage. Avoid repeated freeze-thaw cycles. The expiry date is specified on the product label.
How do you make a hygromycin B stock solution?
Preparation Instructions Hygromycin B is soluble in water at >50 mg/mL. 2 It is also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Hygromycin B is dissolved in water at 50 mg/mL, then sterile-filtered to produce H 0654, or γ-irradiated to produce H 5527.
How is nalidixic acid stock solution prepared?
Weigh 0.30 g of Nalidixic Acid (Nalidixic Acid, GoldBio Catalog # N-015 [CAS 389-08-2, mw. = 232.24 g/mol]). 2. Add 10 ml of 0.3M NaOH and dissolve completely.
How do you make a spectinomycin stock solution?
Weigh 0.5-1 g of spectinomycin [Spectinomycin /Dihydrochloride Pentahydrate GoldBio Catalog # S- 140, (CAS 21736-83-4, MW = 495.3 g/mol)]. 2. Add 10 ml of sterile H2O. Dissolve completely.
How do you dissolve nalidixic acid?
Solvent used for preparation of stock solution of nalidixic acid: 1/2 volume of water, then add 1mol/L NaoH dropwise to dissolve. Thank you so much for your answer , will surely try it. The acid is not soluble in water (0.1 mg/mL) but the sodium salt is (100 mg/mL).
How do you make antibiotic stock?
Preparation of 80ml stock solutionTetracycline is kept in the 4C fridge in 68-564D. ... Weigh 400mg of tetracyline HCL into a small weigh boat.Dilute 95% Ethanol to 70% using milliQ water. ... Add 80ml of 70% Ethanol to a 250ml bottle.Add the tetracycline HCL to the ethanol.More items...
How do I make a 50 ug ml solution?
So convert 100 mg/mL into ug/mL = 100,000 ug/mL. You will now see that your stock requires a 10000x dilution to reach 10 ug/mL and will require a 2000x dilution to reach 50 ug/mL. You can use c1v1=c2v2 to determine the volumes required to perform the dilutions.
How do you make 100 mg/ml ampicillin?
Dissolve 1 g of sodium ampicillin in sufficient H2O to make a final volume of 10 mL. If sterilization is required, prewash a 0.45- or 0.22-μm sterile filter by drawing through 50-100 mL of H2O. Then pass the ampicillin solution through the washed filter.
How do you dissolve chloramphenicol in water?
Add 10 ml of 95% EtOH (70% may also be used). Dissolve completely.
Hygromycin B Gold: an ultrapure Hygromycin B, endotoxin tested
Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
Specifications
Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Details
Hygromycin B Gold is normally used at a concentration of 200 μg/ml, a 500-fold dilution from the stock solution. However, the optimal concentration needs to be determined for your cells. Suggested concentrations of Hygromycin B Gold for selection in some examples of mammalian cells are listed below:
FAQs
In the TDS it is stated that less Hygromycin B Gold (50-100 µg/ml) can be used in low salt LB, however we only have premixed high salt LB in our lab, therefore what concentration of Hygromycin B Gold should be used for selection? We recommend to use Hygromycin B Gold at a concentration between 100 – 150 µg/ml for high salt LB conditions.
Mechanism of resistance
Resistance genes are kinases that inactivate Hygromycin B through phosphorylation.
Usage notes
The working concentration for selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for resistant cells varies between 25 and 1000 μg/ml. You need to determine your optimum concentration experimentally. For some typical values by cell types see the table below.
