Ampicillin - 10 mL (1,000 X Stock)
- Dissolve 1 g of ampicillin into 10 ml of ddH 2 O.
- Filter through a 0.22 µm filter to sterilize.
- Aliquot and store at -20°C.
- Use at 1:1000 dilution in LB or LB-Agar.
How do you prepare a stock solution?
- A very accurate standard alkaline solution cannot be prepared using sodium hydroxide as the primary standard. ...
- Pure anhydrous sodium carbonate, Na 2 CO 3, is used to prepare a primary standard alkaline solution.
- Solid organic acids such as oxalic acid, H 2 C 2 O 4 .2H 2 O, is used to prepare primary standard acidic solution.
How to prepare a solution from stock solution?
Example of How to Prepare a Solution
- Weigh out 58.44 g NaCl.
- Place the NaCl in a 1-liter volumetric flask.
- Add a small volume of distilled, deionized water to dissolve the salt.
- Fill the flask to the 1 L line.
How to make stock solution?
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How to make ampicillin stock?
Ampicillin Stock (1000x, makes 10 vials) 1. Take Ampicillin powder from the -20°C and weigh out 1 g on the analytical balance. 2. Place the weighed amount into a 15 mL conical vial that has 10 mL of water in it. 3. Shake vigorously until all is dissolved. 4. Obtain a 12 mL sterile syringe and .2 um sterile filter. 5. Obtain a second 10 mL centrifuge tube. 6. Clean and sterilize the hood. 7 ...
How do you make 1000X ampicillin stock?
1) Dissolve 1 g of ampicillin into 10 ml of ddH2O. 2) Filter through a 0.22 µm filter to sterilize. 3) Aliquot and store at -20°C. 4) Use at 1:1000 dilution in LB or LB-Agar.
How do you make antibiotic stock solution?
Preparation of 80ml stock solutionTetracycline is kept in the 4C fridge in 68-564D. ... Weigh 400mg of tetracyline HCL into a small weigh boat.Dilute 95% Ethanol to 70% using milliQ water. ... Add 80ml of 70% Ethanol to a 250ml bottle.Add the tetracycline HCL to the ethanol.More items...
How do you prepare 100mg ml ampicillin?
Dissolve 1 g of sodium ampicillin in sufficient H2O to make a final volume of 10 mL. If sterilization is required, prewash a 0.45- or 0.22-μm sterile filter by drawing through 50-100 mL of H2O. Then pass the ampicillin solution through the washed filter.
How do you make 50mg ml ampicillin?
A 50 mg/ml stock solution of ampicillin can be prepared by dissolving 500 mg ampicillin sodium salt in water to a final volume of 10 ml.
How do you make 1000x antibiotic stock?
Preparation of 1000X Antibiotic Stock SolutionWeigh x grams of antibiotic-of-interest.Dilute in pure 100% ethanol for chloramphenicol or sterile deionized water for kanamycin and ampicillin.Syringe filter water-based antibiotic solution using a 0.22-μm filter.
How do you make ampicillin stock from powder?
1. Mix 2 g Amp powder into 20 mL of MilliQ H2O. 2. Vortex until powder is thoroughly dissolved.
How do you dissolve ampicillin in water?
Ampicillin available from Sigma A-9518 (Ampicillin sodium salt), FW 371.39. To make 100ml of 100 mg/ml stock solution, dissolve 10 g of ampicillin in 50 ml of water and 50 ml of 100% ethanol.
How much ampicillin is in a pound of broth?
Ampicillin is used at a final concentration of 50 µg/ml in both LB-Ampicillin broth and LB-Ampicillin agar. Ampicillin should be stored at –20° and is good for 1 year.
How do you store ampicillin stock?
Culture plates with ampicillin can be stored at 2-8 °C for up to two weeks. Stock solutions may be stored at 2-8 °C for up to 3 weeks. For long term storage (4-6 months), stock solutions should be stored at -20 °C. At 37 °C in culture, ampicillin is stable up to 3 days.
How do you prepare 50mg ml?
How to make a 50 mg/ml Kanamycin Stock SolutionAdd 10 ml of sterile H2O. Disolve completely. 00:02:00.Prewet a 0.2 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water. 00:02:00.Sterilize Kanamycin Stock through the prepared 0.2 µm syringe filter. Stock may be kept at -20°C for 1 year. 00:02:00.
How do you dissolve ampicillin powder?
As Michael said, Ampicillin sodium salt is quite soluble in water, yet Ampicillin Anhydrous is not. I would recommend using the sodium salt version if you can, as it will make your life quite a bit easier. I have made an Ampicillin Anhydrous Stock Solution, and it required adding a fair bit of 1 M HCl acid to dissolve.
How do I prepare antibiotic stock solution? - ResearchGate
I want to prepare antibiotic stock solution of Amikacin. But they have given the potency between 674-786 µg/mg. How should I calculate the exact potency for preparing stock solution?
Ampicillin stock solution (100 mg/mL) - CSH Protocols
How to effectively dissolve Ampicillin to enable it to filter sterilize?
Ampicillin (Amp) Stock Preparation · Benchling
Guide To Preparation of Stock Standard Solutions
Most recent answer
I usually pour the Amp powder on the water. It dissolve very well. pouring water on the powder makes it unsoluble. and also I have seen some protocols using half water and half Et-OH.
Popular Answers (1)
Sodium ampicillin is fairly soluble in water and should be fine at that concentration. However ampicillin trihydrate or ampicillin anhydrous is much much less soluble, I think the limits are around 5-10 mg /ml. I'm guessing you are not using the sodium salt so yes if you want that concentrated of a stock solution you need to add NaOH.
All Answers (7)
Ampicillin is (slightly) soluble in water, you should be able to achieve 100 mg/ml, thats what our lab used for stocks, however Sigma here: https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/a9393pis.pdf is recommending a 50 mg/ml solution in water, so try that.
