Full Answer
How do you prepare 50 milliliters of a stock solution?
As an example, say you need to prepare 50 milliliters of a 1.0 M solution from a 2.0 M stock solution. Your first step is to calculate the volume of stock solution that is required. To make your solution, pour 25 ml of stock solution into a 50 ml volumetric flask.
How much volume should be taken from the stock solution?
Add your answer and earn points. 9.17 mL of the stock solution should be taken and diluted upto 110 mL to prepare the 0.500 M solution In this question we are given the volume taken from the stock solution , we have to find the concentration of the diluted solution
How do I make a stock solution?
Your first step is to calculate the volume of stock solution that is required. To make your solution, pour 25 ml of stock solution into a 50 ml volumetric flask. Dilute it with solvent to the 50 ml line.
How do you dilute a concentrated stock solution?
Make sure you pour the concentrated solution into the flask and then dilute it to the volume mark. Do not, for example, mix 250 ml of concentrated solution with 1 liter of solvent to make a 1-liter solution. Helmenstine, Anne Marie, Ph.D. "Dilution Calculations From Stock Solutions."
How do you calculate how much stock solution you need?
To determine the volume of stock solution needed, divide the number of moles of glucose by the molarity of the stock solution.
How do you make a mL from a stock solution?
To prepare the 10 mL of 2 M solution, you must first transfer about 5 mL of distilled water into your 10 mL volumetric flask. Next, slowly add your 4 mL of stock solution (sulfuric acid). Swirl the flask and then top it up with more distilled water to the 10 mL mark.
What does 1X stock solution mean?
• Some stock solutions are concentrated and need to be diluted before using. • Form example, a 10X stock solution is one that contains ten times the concentration of all solutes relative to a working solution, which is considered to be a 1X solution.
How concentrated is a stock solution?
Stock solutions do not necessarily come in concentrations of simple numbers; for example a solution could be 0.1 M HCl. In biochemistry, the term is often used to refer to a concentrated solution, from which one can dilute into a working concentration of solution.
How do you make a 100 mM stock solution?
For practical purpuse it is best to prepare a concentrated stock solution. 416.6 mg in 10 ml will give you 100 mM solution. This can be aliquoted into multiple vials and kept frozen. When needed, thaw one vial and then use this stock soluition to make further dilutions.
How do you make a 1mg ml stock solution?
To prepare a 1 mg/ml stock solution, add 100 mg of the Benzylaminopurine to a 100 ml volumetric flask or other glass container. 2. Add 2-5 ml of 1 N NaOH to dissolve the powder. Once completely dissolved, bring to volume with molecular biology grade water.
What does 1000x Stock mean?
In simpler words, the 1000x return is the amount you receive after selling the crypto having 1000x value. This amount is found to be exactly or approximately a thousand times the original value. Also, it can be said that for every one dollar you invest you will get a thousand dollars after some time.
What does 50X stock solution mean?
X means 'times' or multiplication. 50X TAE is 50 times as concentrated as 1X TAE. do you see? so, you add 50 times as much stuff to the same amount of water (with correct molar ratios) to achieve a 50X solution.
How do you make a 1X stock solution?
For example: A stock solution that is 500 times more concentration would be written as 500X and would require a 1:500 dilution to restore the normal working concentration. Therefore, to make a 1X working solution you would need to add 2 µL of your stock solution into 1 mL of a buffer of your choice.
How do I make a 10 mg/ml stock solution?
To prepare a concentration of 10 µg/ml, pipette out 10 µl of the drug in a test tube using a micropipette. Then dilute it with 990 µl (making a total volume of 1 ml) of the solvent (ethanol, methanol, water etc) you are going to use in your experiment. Similarly, do for 20 µg/ml.
How do you make a stock solution?
A stock solution is prepared by weighing out an appropriate portion of a pure solid or by measuring out an appropriate volume of a pure liquid, placing it in a suitable flask, and diluting to a known volume. Exactly how one measure's the reagent depends on the desired concentration unit.
What is meant by a stock solution?
Definition of stock solution : a concentrated solution (as of developer) that usually is diluted with water before use in photography.
What is solution A?
A is a solution of trioxonitrate (v)acid, HNO3 of unknown concentration.B is standard solution of sodium hydroxide containing 4.00g per dm3 of solution. 25 cm3 portion solution B required an average of 24.00cm3 of solution A for
What is the difference between solution A and B?
A is a solution of trioxonitrate (v) acid , HNO3, of unknown concentration. B is standard solution of sodium hydroxide containing 4.00g per dm3 of solution. 25cm3 portions solution B required an average of 24.00cm3 of solution A for.
How many milliliters of alcohol does Deon use?
1. In the lab, Deon has two solutions that contain alcohol and is mixing them with each other. Solution A is 40% alcohol and Solution B is 12% alcohol. He uses 1000 milliliters of Solution A. How many milliliters of Solution B
What is a dilute solution?
A dilution is a solution made by adding more solvent to a more concentrated solution (stock solution), which reduces the concentration of the solute. An example of a dilute solution is tap water, which is mostly water (solvent), with a small amount of dissolved minerals and gasses (solutes).
Why do you start with a concentrated solution and then dilute it to make a dilution?
The primary reason you start with a concentrated solution and then dilute it to make a dilution is that it's very difficult—and sometimes impossible—to accurately measure solute to prepare a dilute solution, so there would be a large degree of error in the concentration value.
Can you dilute a solution with too much solvent?
It's a common mistake to add too much solvent when making the dilution. Make sure you pour the concentrated solution into the flask and then dilute it to the volume mark. Do not, for example, mix 250 ml of concentrated solution with 1 liter of solvent to make a 1-liter solution.