Add 1.4286 milliliter of water to make the stock solution 2. If you can't add add the above mentioned amount of water then you can calculate the molarity again and it would be 14.2857 millimolar. now you can dilute this solution to 10 mM by using M1V1 = M2V2 formula your stock molarity is 14.2857 millimolar
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How much antibiotic is in a stock solution?
Stock solutions are typically at 100 mg/ml, so that 1 ml of antibiotic can be added to 1 liter of broth or agar. Stock solutions made in 50% alcohol remain liquid at -20°C and are easy to pipet.
What is the concentration of ampicillin in a stock solution?
Working Solution: Typical concentrations of ampicillin are 50 μg/mL for low copy plasmids and 100 μg/mL for high copy plasmids. Stock solutions are typically at 100 mg/ml, so that 1 ml of antibiotic can be added to 1 liter of broth or agar. Stock solutions made in 50% alcohol remain liquid at -20°C and are easy to pipet.
How much salt is required to increase the potency of antibiotics?
As Mukesh says, the salt-containing formulation is not going to affect your antibiotic's potency. For your requirement, from what you have mentioned above, it is evident that you need a working concentration of 10ug/mL or 10 ppm (10mg/L).
What is the concentration of ampicillin and gentamicin sulfate in culture broth?
I have used ampicillin and Kanamycin for my research; the final concentrations (to be used in the culture) would be 50 ppm ampicillin and 75ppm ampicillin in my culture broth. You need to take 10x1.081=10.81mg of gentamicin sulfate. Good luck! For a working concentration of 10 ug/ml, you need to prepare a stock of 10 mg/ml.
How do you make an antibiotic stock solution?
Preparation of 80ml stock solutionTetracycline is kept in the 4C fridge in 68-564D. ... Weigh 400mg of tetracyline HCL into a small weigh boat.Dilute 95% Ethanol to 70% using milliQ water. ... Add 80ml of 70% Ethanol to a 250ml bottle.Add the tetracycline HCL to the ethanol.More items...
How do you make 1000X antibiotic stock?
Preparation of 1000X Antibiotic Stock SolutionWeigh x grams of antibiotic-of-interest.Dilute in pure 100% ethanol for chloramphenicol or sterile deionized water for kanamycin and ampicillin.Syringe filter water-based antibiotic solution using a 0.22-μm filter.
How do you make 50 mg/ml stock solution?
How to make a 50 mg/ml Kanamycin Stock SolutionAdd 10 ml of sterile H2O. Disolve completely. 00:02:00.Prewet a 0.2 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water. 00:02:00.Sterilize Kanamycin Stock through the prepared 0.2 µm syringe filter. Stock may be kept at -20°C for 1 year. 00:02:00.
How do you make cephalexin stock solution?
stock solution: 20 mg/ml in ~60mM NH4OH. Suspend 20g cephalexin in 900 ul water, then add ~60 ul 1M NH4OH.
What does 1000x stock mean?
1000x concentration just means your stock is 1000x more concentrated than your working concentration. For example, if your stock is 50 mg/mL the working concentration is 50 ug/mL. To go from one to the other you dilute your stock 1000x, so for 1.5 mL you would add 1.5 uL.
How do you make 100 mg/ml ampicillin stock solution?
Dissolve 1 g of sodium ampicillin in sufficient H2O to make a final volume of 10 mL. If sterilization is required, prewash a 0.45- or 0.22-μm sterile filter by drawing through 50-100 mL of H2O. Then pass the ampicillin solution through the washed filter.
How do you make 5 mg/ml stock solution?
All Answers (7) Somayeh, Dissolve 5 gram in 1000 ml : 5 mg /ml, Then 0.5 gram in 1000 ml : 0.5 mg /ml. in order to prepare the various concentration you must first prepare a stock solution of your plant extract. prepare the the stock by dissolving 0.5 gm in solvent.
How do you calculate how much stock solution you need?
To determine the volume of stock solution needed, divide the number of moles of glucose by the molarity of the stock solution.
How do you calculate the dilution of a stock solution?
To make a fixed amount of a dilute solution from a stock solution, you can use the formula: C1V1 = C2V2 where: V1 = Volume of stock solution needed to make the new solution. C1 = Concentration of stock solution.
What is the stock solution of antibiotic?
Antibiotic stock solutions are concentrated solutions from which a working concentration (that is more diluted in form than the stock solution) can be obtained. The antibiotic stock solution of a particular antimicrobial agent is more concentrated than the actual working concentration of the same antibiotic.
How do you make cefotaxime stock solution?
Weigh 1 g of Cefotaxime (Cefotaxime (Sodium), GoldBio Catalog # C-104 [CAS 64485-93-4, mw. = 477.5]). 2. Add 10 ml of sterile H2O.
How do you make ciprofloxacin stock solution?
Dissolve 25 mg of ciprofloxacin powder into 25 mL of DEPC water to make 1 mg/mL ciprofloxacin stock solution.
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Dear and respected all researchers, I have an article with procedure using 1g/ L of cefotaxime mentioned, what did it mean?
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Thank You for your kind reply. But when we don't know the molecular weight how we can say or justify 4 ml contains 0.8 mg. Why because we are dissolving total Avagadro number in 4 ml. I am not sure about the answer as I am not good in basics.
Popular Answers (1)
No need of potency here as you intend to assay mass concentrations (btw there must be something wrong in your calculation as your calculated unit is uncompatible with the potency...). 20x stock should be 2.56mg/mL. Te important thing to know about the starting material is it's actual content in colistin (when pure it's 30000U/mg).
All Answers (6)
No need of potency here as you intend to assay mass concentrations (btw there must be something wrong in your calculation as your calculated unit is uncompatible with the potency...). 20x stock should be 2.56mg/mL. Te important thing to know about the starting material is it's actual content in colistin (when pure it's 30000U/mg).
Similar questions and discussions
My antibiotic is Colistin Sulfate Salt given with 15000 Units / mg. I need to prepare 20 ug / ml. Please anyone can help me with the calculation?
